Relationship between effect of WEPO on tracheal smooth muscle andβand M receptors / 军事医学

ABSTRACT

Objective To study the anti-asthma mechanism of water extract of Portulaca oleracea L( WEPO) related toβand M receptors.Methods Rabbits of 2.0 -2.5 kg were selected,whose isolated tracheal rings were prepared.The effect of WEPO on tracheal smooth muscle tension was evaluated using tracheal smooth muscle contraction frequency and contraction amplitude as indexes.Aminophylline( Ami) was used as a positive control medicine and propranolol( Pro) as theβreceptor blocking agent to observe the relaxation effect on tracheal smooth muscle of WEPO related to βreceptors. Acetylcholine chloride( AChCl) was used as the agonist for M receptors and atronine( Atr) as the M receptor blocking agent to observe the relaxation effect on tracheal smooth muscle of WEPO related to M receptors.Results ①The contraction frequency and amplitude of isolated tracheal smooth muscle in each dose group of WEPO were significantly lower after treatment.The difference was statistically significant(P 0.05 ) .⑤These indexes in Atr+WEPO group were not different those in WEPO group ( P >0.05 ) .Conclusion WEPO has relaxation effect on isolated tracheal smooth muscle of rabbits,which is related toβreceptors rather than M receptors.