Research advances in quantitative detection of single cell protein / 中国药科大学学报
Journal of China Pharmaceutical University
; (6): 521-531, 2015.
Article
em Zh
| WPRIM
| ID: wpr-481904
Biblioteca responsável:
WPRO
ABSTRACT
Proteins presence and differences of the expression level can clarify the physiological or pathological changes in organisms;so the quantitative detection of proteins is vital for disease mechanism research;diagnosis and prognosis evaluation.Traditional protein quantitation methods at the tissue level reflected the average protein expression in cells;but ignore the differences between individual cells.In contrast;approaches for quantitative detection at single-cell level can better reflect the differences.Recently;a number of approaches for such detec-tion have been proposed;including microfluidics;microwell-based technology;optical fiber nanobiosensor;activity-based probe technology and mass spectrometry.The principles;advantages and drawbacks of these approaches are briefly introduced in this review.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Journal of China Pharmaceutical University
Ano de publicação:
2015
Tipo de documento:
Article