Establishment of a universal fluorescent multiplex cPCR method for detection of copy number variations in mice / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
; (6): 591-596, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-484172
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish a high throughput general multiple competitive polymerase chain reaction ( cPCR) detecting method of copy number variations ( CNVs) for the population of chromosome 1 substitution strains from wild mice.Method The selected 14 loci, including 11 CNVs on chromosome 1 and internal control loci on other three chromosmes (Chr 7, Chr 19 and Chr X), were detected based on the universal fluorescent primer multiple competitive pol-ymerase chain reaction.All specific cloned plasmids were constructed as competitors.Results Altogether 11 CNVs were designed in one panel, and the copy of Chr X accurately reflects the gender.Conclusions A rapid and high-throughput fluorescent multiplex cPCR assay is established which can be used for detection of copy number variations on chromosome 1 in mice.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Acta Laboratorium Animalis Scientia Sinica
Ano de publicação:
2015
Tipo de documento:
Artigo