Effects of Sedum Sarmentosum Total Flavonoids on the Expression of TGF-β1 and Smad 7 in Rats with He-patic Fibrosis / 中国药师

ABSTRACT

Objective:To investigate the inhibitory effects of Sedum Sarmentosum total flavonoids ( SSTF) on hepatic fibrosis in-duced by CCl4 ,and examine the effects on the expression of TGF-β1 and Smad7. Methods:Sixty male SD rats were randomly divided into the normal control group, the model group, 100, 200 and 400 mg·kg-1 SSTF groups and colchicine positive control group. The experimental model of hepatic fibrosis in rats was established by the injection of CCL4 . The liver histopathology was examined by Mas-son stain, and the protein expression and mRNA of TGF-β 1 and Smad7 were assessed by RT-PCR and Western blotting. Results:Compared with the model group, every SSTF group could significantly reduce the degree of liver fibrosis induced by CCL4(P<0. 05). The middle and high dose SSTF gouprs could significantly reduce the protein expression and mRNA of TGF-β1 (P<0. 05), and sig-nificantly increase the protein expression and mRNA of Smad 7 (P<0. 05). Conclusion:SSTF exhibits anti-hepatic fibrosis effects in rats through down-regulating the expression of TGF-β1 and up-regulating the expression of Smad7 in fibrotic liver tissue.