Effect and mechanism of transcription factor Foxp3 on proliferation and cell cycle of human lung adenocarcinoma cell A549 / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 490-494, 2016.
Article
em Zh
| WPRIM
| ID: wpr-485946
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To investigate the effect and mechanism of transcription factor Foxp 3 on the proliferation and cell cycle of human lung adenocarcinoma cell line A 549.Methods:We knocked down the expression of Foxp 3 using siRNA.Foxp3 inhibition was detected by RT-PCR.Cell proliferation was detected by MTT.Cell cycle of A549 cells were detected by flow cytometry after the transfection of siRNA.Cell cycle-related checkpoint genes were filtered by RT-PCR.The regulation of Foxp3 on cell cycle-related checkpoint genes were detected by immunofluorescence and dual -luciferase reporter assay system.Results: The proliferation of A549 cells were inhibited after silencing Foxp3,and A549 cells were arrested in G0/G1 cycle.G1/S cycle checkpoint gene CCND1 was down regulated.Mechanism research show that Foxp 3 can regulate the expression of CCND 1 directly.Conclusion: Foxp3 can promote the proliferation of A549 cell line by up regulating G 1/S cycle checkpoint gene CCND 1.This provides a new target for the therapeutic targets of lung adenocarcinoma.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Ano de publicação:
2016
Tipo de documento:
Article