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Separation and Enrichment of Antibacterial Effective Fraction of Syringae Folium by Macroporous Resins / 中国药师
China Pharmacist ; (12): 437-439, 2016.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-487718
Biblioteca responsável: WPRO
ABSTRACT

Objective:

To investigate the purification of antibacterial effective fraction of Syringae folium by macroporous resins.

Methods:

Static adsorption and desorption tests were carried out to screen the macroporous resins. The desorption experiment was per-formed on the selected D101 resin to optimize the separation process. The effects of resin amount, diameter length ratio, elution flow rate, elution solution concentration and volume were studied.

Results:

The optimal conditions were as followsthe elution solution was 55% ethanol, the adsorption flow rate was 1 BV·h-1 , the elution flow rate was 5 BV·h-1 , 6 BV 25% ethanol was used to eliminate impurity and 8 BV 55% ethanol was used to elute to obtain the effective fraction.

Conclusion:

The content of antibacterial effective component is above 65% after purified by D101 resin, indicating that the present method is suitable for large-scale preparation of anti-bacterial effective fraction of Syringae folium.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2016 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2016 Tipo de documento: Artigo
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