The influence of high-voltage electrical burn on the thrombomodulin, protein C, protein S, and D-Dimer in SD rats / 实用医学杂志

ABSTRACT

Objective To investigate the influence of high-voltage electrical burn on the throm-bomodulin (TM), protein C (PC), protein S (PS) and D-Dimer (D-D) in SD rats. Methods One hundred and twenty healthy SD rats were divided into the fake high-voltage electrical burn groups (FHEB), high-voltage electrical burn groups (HEB) according to the random number table, with 60 rats in each group. Ten rats were taken from each group at 15 minutes before injury. Plasma were collected from heart blood. Fifty SD rats of HEB group with voltage regulator and experimental transformer. The remaining fifty SD rats of FHEB group were sham injured with the same devices without electric current. At 5 minutes and 1, 2, 4, 8 hour (s) post injury, 10 rats of every group were randomly chosen at each time point for observation of the concentrations of TM, PC, PS and D-D. Plasma were collected from heart blood. Data were processed with analysis of variance of factorial design and LSD test. Results Compared with the FHEB group, the concentration of TM from 5 minutes to 8 hours post injury in HEB group was higher significantly (P < 0.05). Exception of the concentrations of PC and PS at 15 minutes before injury, the concentrations of PC and PS were lower than those of FHEB group (P < 0.05). The concentration of D-D in HEB group peaked at 8 hours post injury in (173.05 ± 4.08) ng/mL. Conclusion High-voltage electrical burn at early stage can increase the concentrations of TM, D-D, as well as decrease the concentrations of PC and PS, which are not only causing the vascular endothelium damage but also possessing serious effect on the thromboplastin function of SD rats.