Mutations of S protein gene in positive HBsAg and anti-HBs patients with chronic hepatitis B virus infection / 中华临床感染病杂志
Chinese Journal of Clinical Infectious Diseases
; (6): 533-537, 2015.
Article
em Zh
| WPRIM
| ID: wpr-488663
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate mutations of S protein gene in positive HBsAg and anti-HBs patients with chronic hepatitis B virus (HBV) infection.Methods Fifteen HBsAg(+) and anti-HBs(+) patients and 22 HBsAg(+) and anti-HBs (-) patients (control group) admitted in Renmin Hospital of Wuhan University during January and December 2011 were enrolled in the study.The S protein gene was amplified and sequenced, and the amino acid sequences were translated from the obtained DNA sequences and compared with the reference sequences.Results Compared with the control group, HBsAg (+) and anti-HBs(+) patients showed a higher variability in amino acid within major hydrophilic region (2.95 vs.0.78,x2 =18.059, P<0.01) and the a determinant (4.44 vs.1.52, x2 =6.985, P<0.01).The mutations in a determinant at positions P127T, G130E, G130N, M133S, F134I, T140I and G145R were detected only in HBsAg(+) and anti-HBs (+) patients.Conclusion Co-existence of HBsAg and anti-HBs in patients with chronic HBV infection might be associated with the increased amino acid mutations in and around the a determinant of protein S.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Clinical Infectious Diseases
Ano de publicação:
2015
Tipo de documento:
Article