Downregulation of Ro60 inhibits migration and invasion of human lung adenocarci-noma A549 cells / 军事医学
Military Medical Sciences
; (12): 213-216, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-490765
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the effect of Ro60 on migration and invasion of lung adenocarcinoma A 549 cells. Methods We knocked down Ro60 and analyzed the ability of migration and invasion of A 549 cells.Quantitative real time ( RT)-PCR and Western blotting were performed to detect mRNA and protein levels of selected molecules associated with migration and invasion of neoplasm .Results When Ro60 was downregulated , the migration and invasion of A 549 cells decreased markedly.Meanwhile,the mRNA expression of matrix metalloproteinase (MMP)9,c-Src etc was observably reduced.Furthermore, downregulation of Ro60 diminished the expression of Src protein and the activation of MMP-9 protein.Conclusion Downregulation of Ro60 inhibits migration and invasion of A549 cells by regulating Src protein and activating MMP-9 protein.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Military Medical Sciences
Ano de publicação:
2016
Tipo de documento:
Artigo