Determination of Chlorogenic Acid and Rutin in Tibetan Medicine Nettle by HPLC / 中国药师
China Pharmacist
; (12): 1409-1410, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-495156
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To establish a method to determine the content of chlorogenic acid and rutin in nettle to assess the quality of nettle.Methods:
An HPLC method was used with the following chromatographic conditionsCAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) was used with the mixture of 0.4%phosphoric acid and methanol as the mobile phase with gradient elution , the de-tection wavelength was set at 340 nm, the flow rate was 1.0 ml· min-1 and the sample size was 10μl.Results:
Chlorogenic acid with-in the range of 2.360-23.600μg · ml-1 showed a good linear relationship (r=0.999 9), and rutin within the range of 6.208-62.080μg · ml-1 showed a good linear relationship (r=0.999 9).The recovery of rutin and chlorogenic acid was 99.20% and 100.40%with RSD of 1.2%and 1.1%(n=6), respectively.Conclusion:
The method is fast, effective, simple, accurate and reproducible , which can be used to quantitatively analyze chlorogenic acid and rutin in nettle .
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2016
Tipo de documento:
Artigo