Study on Standard Revision of Portulaca grandiflora / 中国药房
China Pharmacy
; (12): 2980-2982, 2015.
Article
em Zh
| WPRIM
| ID: wpr-500801
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:To improve the quality standard of Portulaca grandiflora. METHODS:TLC was used to identify the P. grandiflora with the reference of letuolin and apigenin. HPLC was performed on the column of Hanbon-C18 with the mobile phase of methanol-acid gradient[water-acetic acid(61∶4)] at the flow rate of 1.0 ml/min,the detection wavelength was 335 nm,the temperature was 35 ℃ and the volume was 10 μl. RESULTS:There was a good linear relationship in the quality concentration of scutellarin and luteolin respectively in th range of 0.299 5-1.872 0 μg(r=0.999 8)and 0.015 1-0.094 4 μg(r=0.999 5);the RSDs of precision,stability and reproducibility tests were ≤2.61%. The average recovery was respectively 100.42%(RSD=0.81%,n=6) and 100.65%(RSD=1.64%,n=6). CONCLUSIONS:The established TLC and HPLC are stable,reliable,simple and can be used for the qualitative and quantitative analysis of P. grandiflora.
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Base de dados:
WPRIM
Tipo de estudo:
Qualitative_research
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2015
Tipo de documento:
Article