Contents Determination of 7 Ingredient in Xianglian Huazhi Pill by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for the contents determination of paeoniflorin,hesperidin,baicalin,palmatine, berberine,glycyrrhizic acid,magnolol in Xianglian huazhi pill. METHODS:HPLC was conducted on the column of Agilent Porlshell 120 SB-C18 with mobile phase of 90% acetonitrile(A)-0.1% phosphoric acid 5% aqueous acetonitrile(B)(gradient elu-tion)at a flow rate 1.0 ml/min,the column temperature was 30 ℃,the injection volume was 3 μl,and the detection wavelength was 230 nm for paeoniflorin(0-10.0 min),283 nm for hesperidin(10.1-13.0 min),277 nm for baicalin(13.1-14.4 min),265 nm for palmatine and berberine (14.5-20 min),250 nm for glycyrrhizic acid (20.1-25.0 min) and 290 nm for magnolol (25.1-60.0 min). RESULTS:The linear range was 0.013 88-0.694 5μg for paeoniflorin,0.039 93-1.996 8μg for hesperidin,0.070 08-0.389 3μg for baicalin,0.006 48-0.324 0μg for palmatine(counted by palmatine hydrochloride),0.010 52-0.526 2μg for berberine(counted by berberine hydrochloride),0.008 816-0.440 7 μg for glycyrrhizic acid(counted by ammonium glycyrrhizinate)and 0.007 224-0.361 2 μg for magnolol(r≥0.999 0);RSDs of precision,stability and reproducibility tests were lower than 2.0%;average recoveries were 97.47%-102.88%(RSD=1.91%,n=6),102.06%-102.81%(RSD=0.25%,n=6),97.91%-100.80%(RSD=1.25%,n=6), 97.53%-101.60%(RSD=1.66%,n=6),97.54%-100.68%(RSD=1.15%,n=6),96.23%-99.00%(RSD=1.11%,n=6) and 97.91%-101.44%(RSD=1.36%,n=6),respectively. CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the quality control of Xianglian huazhi pill.