Construction and characterization of RNAi lentiviral vector targeting human CD106 gene / 实用口腔医学杂志
Journal of Practical Stomatology
; (6): 783-786, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-506249
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To construct CD106-targeted RNAi lentiviral vector plasmids.Methods:
4 targets aimed at CD106(Target 1, 2, 3, 4)were designed. Oligo-DNA fragment containing short hairpin frame was synthesized and reannealed, and then cloned into lentivi-ral expression vector. PCR and sequencing analysis were made for verifying the positive clones. The virus packaging plasmids were trans-fected into 293T cells to harvest siRNA lentivirus. After infection in HN12 cells, Real-time PCR and western blot were performed to de-termine the expressing level of CD106.Results:
PCR and sequencing revealed that siRNA plasmids was correctly constructed. Virus with a titer of 1 × 109 TU/ml was successfully packaged at least. CD106 expression in HN12 cells could be knockdown by virus infection sig-nifically, compared with negative control lentivirus.Conclusion:
The recombinant lentiviral siRNA expressing vector targeting human CD106 gene has been successfully constructed and packaged. CD106 gene in cells may be down-regulated by lentiviral siRNA.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Practical Stomatology
Ano de publicação:
2016
Tipo de documento:
Artigo