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Mutations in C region of hepatitis B virus may contribute to occult hepatitis B virus infection / 实用医学杂志
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-512795
Biblioteca responsável: WPRO
ABSTRACT
Objective To investigate the role of mutations in C region that may contribute to occult hepatitis B virus infection.Methods C genes were amplified from two OBI blood donor samples respectively.Plasmids with mutations in C region of hepatitis B virus were constructed by overlapping PCR.HBsAg and HBeAg in Huh7 cells and in the serum of Balb/c mice were detected by CMLA.HBcAg in liver tissue was detected by immunohistochemistry,while HBV-RNA was tested by RT-PCR.Results Mutations in C region significantly reduced the expression level of HBeAg and HBcAg,but had no significant effect on HBsAg and HBV-RNA.Conclusion The mutations in C region affect the expression level of HBeAg and HBcAg,which may play an important role in the occurrence of OBI.

Texto completo: Disponível Contexto em Saúde: ODS3 - Saúde e Bem-Estar Problema de saúde: Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: The Journal of Practical Medicine Ano de publicação: 2017 Tipo de documento: Artigo
Texto completo: Disponível Contexto em Saúde: ODS3 - Saúde e Bem-Estar Problema de saúde: Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: The Journal of Practical Medicine Ano de publicação: 2017 Tipo de documento: Artigo
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