Detection of genetic instability in gastric cancer tissues by RAPD PCR / 中国普通外科杂志

ABSTRACT

ObjectiveTo detect the instabilities of DNA and chromosome in gastric carcinoma. Methods A total of 33 gastric cancer specimens were analyzed by RAPD(random amplified polymorphic DNA) PCR with nine 10-base arbitrary primers for detecting instabilities of DNA and chromosome. ResultsSample 5 and 3 showed the highest genomic changes and that there were significant differences in the ability of each primer to detect genomic instability ranging from 21% to 85%.ConclusionsThe genetic instabilities often concentrated on some special loci of chromosome e.g. repetitive sequences. It is difficult to influence the result of cancer treatment in gene therapy targeting at only one oncogene or tumor suppressor gene because of the extensive DNA variations occurred during the progression of tumor.