Effect of tumor necrosis factor ? or angiotensin Ⅱ on tissue factor expression in human vascular endothelial cells / 中国病理生理杂志

ABSTRACT

AIM: To investigate the influence of tumor necrosis factor ?(TNF ?) or angiotensin Ⅱ(Ang Ⅱ) on tissue factor(TF) gene expression and the regulatory effect of upstream sequence on TNF ? or Ang Ⅱ-induced TF gene transcription in human vascular endothelial cells.METHODS: TF mRNA in endothelial cells was analyzed by in situ hybridization. By means of gene recombinant technique, we constructed two luciferase reporter genes containing different upstream sequence of TF gene. The two luciferase reporter genes, together with the intracontrol plasmid pSV-?-gal were co-transfected respectively into cultured human vascular endothelial cells. The relative luciferase activities were detected.RESULTS: TNF? or Ang Ⅱ could increase the expression of TF mRNA in vascular endothelial cells. TNF? and AngⅡ could significantly increase the luciferase expression in the p-244/+121bp Luc transfected endothelial cells, respectively. The luciferase expression in the p-111/+121bp Luc transfected endothelial cells decreased significantly compared with that in the p-244/+121 bp Luc transfected endothelial cells and there was no significant difference compared with control group. CONCLUSION: Upstream sequence p-244/-112bp of TF gene may play an important regulatory role in TNF ? or AngⅡ-induced TF gene expression in human vascular endothelial cells.