Effects of MMP-7asODN on MMP-7 mRNA expression and invasion of KATOⅢ cell / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-518695
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To investigate the effects of MMP-7asODN on MMP-7 expression in human stomach cancer cell line KATOIII and explore the effect of MMP-7 on cancer invasion and metastasis.METHODS:
A 15mer PS-asODNs targeted against the MMP-7 mRNA were introduced into KATOIII cells by FuGENE TM 6. The levels of MMP-7 mRNA was observed by reverse transcription polymerase chain reaction (RT-PCR). The ability of the invasion was observed by Boyden Chamber invasion assay.RESULTS:
The MMP-7/?-actin ration was 0.31?0.02 for the PS-asODNs treated cells which is significantly lower compared with the control and PS-sODN and PS-mODN( 1.59?0.01, 1.14?0.03,1.51?0.02, respectivly.) P
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo