Interleukine 1 receptor antigonist inhibits proinflammatory factor production by human retinal pigment epithelial cells in vitro / 中华眼底病杂志
Chinese Journal of Ocular Fundus Diseases
; (6)1996.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-518753
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate production of interleukine 6 (IL 6) and interleukine 8 (IL 8) by retinal pigment epithelial (RPE) cells and its inhibition by interleukine 1 receptor antigonist(IL 1ra). Methods Cultured human RPE cells was treated with interleukine 1 ? (IL 1?, 10 ng/ml) and/or IL 1ra ( IL 1ra, 1、10、100 ng/ml). IL 6 and IL 8 mRNA and protein expression were detected by ELISA, immunohistochemistry and in situ hybridization (ISH) assay. Results IL 6 and IL 8 in conditioned media of RPE cells in controls was 2 000 pg/ml and 5 000 pg/ml respectively after stimulation of IL 1? for 8 h. IL 1ra (100 ng/ml) significantly inhibited IL 6 (300 pg/ml, t=8.011, P
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Ocular Fundus Diseases
Ano de publicação:
1996
Tipo de documento:
Artigo