Determination of the Content of Dehydroepiandrosterone in Dehydroepiandrosterone Tablets by HPLC / 中国药房

ABSTRACT

OBJECTIVE:HPLC method was established for the determination of dehydroepiandrosterone(DHEA)in tablets.METHODS:The chromatographic system consisted of ?-Bondapak C18 column and mobile phase of methanol-water(75∶25)with a detection wavelength at 292nm.RESULTS:The linear range of DHEA was 0.2～1.0mg/ml,r=0.9 999.The recovery rate was 99.13%(RSD=0.42%).CONCLUSION:This method is simple and accurate for determination of DHEA in tablets.