Effects of thrombin, arachidonic acid and aspirin on leukocyte-platelet adhesion / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effects of different concentrations of thrombin, arachidonic acid (AA) and aspirin on leukocyte-platelet adhesion. METHODS: The methods of Hamburger et al and Shen Zhi-qiang et al were used to study the adhesion between platelets and leukocytes in rats. RESULTS: 50 U/L of thrombin markedly stimulated the binding between platelets and leukocytes; the efficacy of thrombin increased with its concentration and produced the maximum effect at 300 U/L. But the adhesion rate decreased while the concentration of thrombin was more than 300 U/L. 25 ?mol/L of AA significantly enhanced the binding of platelets to leukocytes; the efficacy of AA increased with its concentration and obtained the maximum effect at 100 ?mol/L. The adhesion rate, however, decreased while the concentration of AA was more than 100 ?mol/L. Aspirin could inhibit thrombin-or AA-induced adhesion between platelets and leukocytes. CONCLUSION: It is suggested that thrombin and AA, in a certain range of concentrations, concentration-dependently induced the binding of platelets to leukocytes; the adhesive rate, however, decreased as the concentration of the above inducers increased. Aspirin could inhibit platelet-leukocyte adhesion stimulated by thrombin and AA.