Determination of Content of Baicalin in Kugan Granules With HPLC / 中国药房

ABSTRACT

OBJECTIVE:To develop a HPLC method for determination of the content of baicalin in Kugan gran?ules.METHODS:ODS-C 18 column was used and methanol-water-acetic acid(45∶55∶1)was used as mobile phase and UV detection wavelength was274nm.RESULTS:There was a good linear relationship between the concentration of Baicalin and absorption area value in range of0.25?g～2.5?g r=0.9992.The average recovery was98.4%with RSD=1.09%(n=5). CONCLUSION:The method was highly repeatable and accurate.