A Competitive RT-PCR to Study Apolipoprotein E Gene Gxpression in Adipose Cells / 中国医师杂志
Journal of Chinese Physician
; (12)2001.
Article
em Zh
| WPRIM
| ID: wpr-521905
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish the method to detect the expression of apolipoprotein E gene in human adipose cells. Methods Apolipoprotein E gene expression was determined by competitive reverse transcription-PCR in adipose cells of healthy children. Results Apolipoprotein E gene expressed in the adipose cells of healthy children. The mean expression quantity of apolipoprotein E mRNA in adipose cells was (0.41?0.12 ) in healthy children. Conclusions Competitive reverse transcription-PCR is a rapid, simple, sensitive and accurate method to analyze expression of apolipoprotein E gene.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Chinese Physician
Ano de publicação:
2001
Tipo de documento:
Article