Reconstructed corneal endothelial tissue on allo-stroma in vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-522772
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
In order to search carrier material and better tissue culture method, the morphology and structure of the cultured cat corneal endothelium was observed.METHODS:
The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, and then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology and structure of reconstructed tissue was tested by the inverted microscope and the scanning electron microscope.RESULTS:
As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemet's membrane, which was similar to the nature tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.CONCLUSION:
The cat corneal endothelial cells could be rebuilt on the carrier of the dehydrated swine corneal stroma successfully, which is similar to the nature tissue.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1989
Tipo de documento:
Artigo