Expression and clinical significance of DNA topoisomerase and glutathione S-transferases in acute leukemia / 中国病理生理杂志

ABSTRACT

AIM: To investigate the relationship between the expressions of DNA topoisomerase (Topo), glutathione S-transferases (GSTs) and chemotherapy response, prognosis in acute leukemia (AL). METHODS: Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of TopoⅠ, Ⅱ?, Ⅱ? and GST?, ? from patients with AL. RESULTS: The results showed that the relative mRNA expression level of TopoⅡ?, TopoⅡ? and GST ? in AL group were significantly higher than that in normal subjects. GST?, however, was exactly reverse ( P