Determination of Concentration of Dextromethorphan Hydrobromide in Human Serum by HPLC / 中国药房
China Pharmacy
; (12)2001.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-523550
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To establish a HPLC method for determination of concentration of dextromethorphan hydrobro?mide in human serum.METHODS:
The assay was conducted on HiQsil C 18 column(4.6mm?150mm,5?m)with acetonitrile-water-acetic acid(35∶63∶2,V/V/V)as mobile phase.The fluorescence detector measured with the excitation wavelength of280nm and the emission wavelength of320nm,and carvedilol was taken as internal standard.RESULTS:
The linear range of calibration curve of dextromethorphan hydrobromide was0.27~21.90ng/ml with a regressive equation of Y=0.1197X+0.0086,r=0.9986.The recovery was high.CONCLUSION:
This method is accurate,rapid and simple,and can be used for de?termining the concentration of dextromethorphan hydrobromide in human serum.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2001
Tipo de documento:
Artigo