The Protective Effects of Nitric Oxide Inhalation on Rabbit Lung Ischemia/reperfusion Injury / 中国医师杂志

ABSTRACT

Objective To investigate the protective effects and mechanism of nitric oxide(NO) inhalation on rabbit lung ischemia/reperfusion injury. Methods Total 32 Japanese white rabbits were randomly divided into four groups, each group containing 8 rabbits. In group A, animals received only thoracotomy. In group B, after animals left chest were opened, their left main pulmonary arteries were occluded for 2h, then reperfused for another 2h. In group C, animal inhaled 20 ppm NO for 30min since the eleventh minute after reperfusion. In group D, animal inhaled 60ppm NO for 30min since the eleventh minutes after reperfusion. The levels of blood PaO 2, cGMP, slCAM-1, XOD and MDA were measured before operation and after reperfusion. The pathologic changes of left lung was observed. The wet/dry (W/D) weight ratio of left lung tissue was measured. Results Both PaO 2 and cGMP levels after reperfusion in group B were much lower than that in groups A, C and D(P