Influence of amylin on apoptosis of human pancreatic islet ?-cells and its molecular mechanism / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-524445
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To investigate the molecular mechanism of amylin in inducing apoptosis of human pancreatic islet ?-cells.METHODS:
Human pancreatic islet cells were isolated and cultured. The cells were treated with amylin or amylin and aminoguanidine (AG group) for 24 h, respectively. Apoptosis of pancreatic islet ?-cells was studied by in situ TUNEL method combined with double staining for insulin and ELISA. The levels of insulin, NO 2 -/NO 3 - and glutathione (GSH), p53 mRNA and bcl-2 mRNA were also detected.RESULTS:
(1) The enrichment factor and the apoptosis rate of pancreatic islet ?-cells in amylin group were markedly higher than that in control group and AG group ( P
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo