Determination of Thioguanosine in Human Red Blood Cells by HPLC / 中国药房
China Pharmacy
; (12)1991.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-527372
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To establish HPLC method for the determination of thioguanosine in human red blood cells.METHODS:
The determination was performed on Hypersil ODS colum. The mobile phase consisted of methanol-water (10∶90) with detection wavelength at 342nm. 6-Thioguanine(6-TG)was generated from thioguanosine through heating and hydrolyzing. 6-TG was extracted into 0.1mol/L hydrochloric acid for sample injection assay with external reference method for the quantification.RESULTS:
The linear range of 6-TG was 30~1 200pmol/8?108RBCs. Its optimum hydrolyzing time was 1 hour and optimal extraction pH value ranged from 11 to 12. The content of phenyl mercury acetate in the extraction solution was 1.3mmol/L.CONCLUSION:
Under optimized conditions, HPLC method for the determination of thioguanosine is fast, accurate and sensitive.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
1991
Tipo de documento:
Artigo