Study on Separation and Isolation of Total Alkaloids from Gelsemium Elegans Benth.by Macroporous Resin / 中国药房

ABSTRACT

OBJECTIVE:To establish a method and a technical process for the purification of total alkaloids from Gelsemium elegans Benth.with macroporous resin.METHODS:Different types of macroporous resin were used to separate and purify total alkaloids,the HPLC peak areas of the main ingredient of the alkaloids form Gelsemium elegans Benth.were compared and the optimum technological parameters were investigated.RESULTS:The HPD-500,HPD-600,HPD-800type macroporous resin showed better comprehensive absorption property,and HPD-800type macroporous resin were used to investigate parameter.CONCLUSIONS:The method of using HPD-800type and eluting with grade ethanol-water can be used to purify the total alkaloids from Gelsemium elegans Benth..