The effect of co-transfection of p53 and angiostatin gene in SG7901 / 中国医师进修杂志
Chinese Journal of Postgraduates of Medicine
; (36)2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-528799
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the co-transfection of p53 and angiostatin gene in the inhibition of SG7901. Methods Transfected the pVITRO2-hp53-hAS into SG7901 with lipofectamine.After transfection, RT-PCR were used to know whether the aimed gene had been transfected and expressed or not. Cell clones trial, MTT growth curve, cell cycle measuring were used to analyze the differences. Results The cells were suppressed by the two genes and inhibition of the combined genes is more powerful than single one. Conclusion The effection of combined genes pVITRO2-hp53-hAS on SG7901 is stronger than either single one. Combined-gene-therapy is a useful anti-carcinoma method.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Postgraduates of Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo