Ventral heterotopic heart transplantation in mice / 中国普通外科杂志

ABSTRACT

Objective To explore an improved technique of mouse ventral heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.Methods The donor heart aorta and the recipient ventral aorta, the donor pulmonary artery and the recipient inferior caval vein, were anastomosed by using the end-to-side suture technique respectively. At the same time, important anatomical structures of transplantation-related sites were measured.Results The diameter of recipient inferior caval vein and ventral aorta was (1.9?0.2)mm and (0.9?0.1)mm respectively. The average length of ventral blood vessels which can be used was (6.0?1.0)mm. The model construction success rate was 92%. The average time of donor operation and recipient operation was (8.0?1.0)min and (40.2?3.0)min respectively. The time of artery anastomosis and vena cava anastomosis was (9.3?1.2)min and (7.8?1.7)min respectively. Conclusions The animal model is stable and can be used for the study of transplantation immunity.