Site specific antibody of Na/Ca exchanger stimulates Na/Ca exchanger current and has cross-reaction with Ca channel and Na pump / 中国病理生理杂志

ABSTRACT

1∶640) was purified by affinity chromatography with a Mab Trap kit.The effects of the purified IgGs on the ion channel and transporter(INa/Ca,INa pump,ICa-L,Itko,Ik1,IK) at concentrations of 10 nmol/L,20 nmol/L and 40 nmol/L were observed by whole cell voltage clamp technique.RESULTS:The purified IgG enhanced INa/Ca,ICa-L and I Na/k pump in a dose-dependent manner and no significant effect on INa,Itko,Ik1 and Ik was found.CONCLUSION:The site specific antibody of Na-Ca exchanger stimulates Na-Ca exchanger current and has cross-reaction with L-type Ca channel and Na pump.