Validation of Determination Method of the Content of Morphine in Compound Glycyrrhiza Oral Solution / 中国药房

ABSTRACT

OBJECTIVE:To validate the determination method of the content of Morphine in Compound Glycyrrhiza oral solution. METHODS: Samples were detected by solid phase extraction-HPLC on Kromasil C8 column (250 mm?4.6 mm,5 ?m) with mobile phase consisted of 0.05 mol?L-1 KH2PO4-0.0 025 mol?L-1 C14H15NaO3S?2H2O-acetonitrile using gradient elution.The detection wavelength was 220 nm and the flow rate was 1.0ml.min-1.RESULTS:The linear range of morphine was 2.02～20.2 ?g?mL-1(r=0.999 7),and the average recovery was 99.0%(RSD=0.40%,n=9).CONCLUSION: The method is accurate,sensitive and stable,and accurate and reliable in determination results.