Isolation of Calcium-Tolerant Cardiomyocytes from Rats / 中国药房
China Pharmacy
; (12)1991.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-531758
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To investigate the method for rapid and effective isolating of calcium-tolerant cardiomyocytes(CTC) from rats.METHODS:
CTC were obtained by retrograde perfusion using modified Langendorff heart perfusion apparatus. After incubation of cardiomyocytes in KB solution, the ion currents were recorded with patch-clamp technique in whole-cell mode.RESULTS:
Of the whole isolated cells, 70% were survival. Of which, 80% were CTC. A typical outward potassium current was recorded.CONCLUSIONS:
A large number of CTC with normal electrophysiological characteristics can be obtained by mastering the digestion time based on variation of perfusion pressure and keeping all the factors in cell isolation under control.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
1991
Tipo de documento:
Artigo