Comparison of Content of Taxol in Stalk, Leaf and Callus of Taxus cuspidata / 中国药房

ABSTRACT

OBJECTIVE: To compare the content of taxol in stalk,leaf and callus of Taxus cuspidata.METHODS:Dichloromethane and water extract were extracted form T.cuspidata using methanol-chloroform(1 ∶ 1).HPLC method was used to determine the content of taxol.RESULTS: The content of taxol in stalk,leaf and callus of T.cuspidata were 0.018 30%,0.001 28%,0.000 66%,respectively.The content of taxol in the callus of T.cuspidata is higher than other two parts of it.CONCLUSION:This study is significance for the development and utilization of T.caspidate and its protection.