Effect of Shouwu Yizhi Capsule on Gene Expression in Notch/Delta Signal Pathway of Vascular Dementia Model Rats / 中医杂志

ABSTRACT

Objective To investigate the effect and mechanism of Shouwu Yizhi Capsule (Capsule of Radix Polygoni Multiflori and Semen Coicis for dementia) on the Notch/Delta signal pathway in experimental rats with vascular dementia (VD). Methods The models were established with the modified Pulsinelli 4 vessel occlusion method. They were randomized into Shouwu Yizhi Capsule group,piracetam group,model group,and sham operation group with 14 rats in each group. Seven days after modeling,the Shouwu Yizhi Capsule group was administered intragastrically Shouwu Yizhi Capsule solution 0.08g/ml,the piracetam group was prescribed intragastrically piracetam solution 0.02g/ml,and the model and sham operation groups were given intragastrically normal saline. Twenty days later,the mRNA expression of HES1,Mash1,and ?-APP on Notch/Delta singal pathway was determined by real-time quantitative fluorescent PCR. Results Compared with the model and piracetam groups,the mRNA expression of HES1 the in Shouwu Yizhi Capsule group was significantly increased (P0.05). Conclusion Shouwu Yizhi Capsule could effectively activate the interaction of HES1,Mash1,and ?-APP on the Notch/Delta signal pathway,being the possible mechanism in the treatment of VD.