DIRECT ELISA FOR THE EARLY DIAGNOSIS OF HFRS / 西安交通大学学报(医学版)

ABSTRACT

The antigen of hemorrhagic fever with renal syndrome (HFRS) virus in the infected suckling mouse brains was purified by a combined method—Protamin sulfate sedimentation and sucrose cushion uhracentrifugation. The Purified antigen was labeled with horseradish peroxidase (HRP). A kind of IgM antibody capture ELISA that used HRP—labeled antigen of HFRS Virus, i.e, direct ELISA, was successfully established for the detection of specific IgM antibody in sera from patients with HFRS. Compared with IgM antibody capture ELISA that used HRP—labeled IgG antibody to HFRS virus ,direct ELISA was similar in sensitivity to it,but direct ELISA could completely avoid the interference caused by rheumatoid factor (RF) as well as could reduce one step immune reaction. 87 serum samples from patients with HFRS(diagnosed clinically) in various stages, including 37 from Patients with HFRS in early stage(from 1 to 5 days after the onset of HFRS)were detected by direct ELISA . The positive rates of specific IgM antibody were 96.5% and 91.8%, respectively. We think that direct ELISA that uses HRP—labeled antigen provides a more specific,simpler and faster method for early di—agnosis of HFRS.