Induced differentiation of rat ectomesenchymal cells to odontoblast-like cells——Setup of three-dimension culture model / 实用口腔医学杂志

ABSTRACT

Objective: To study the mechanism of differentiation of rat ectomesenchymal cells to odontoblasts. Methods: Ectomesenchymal cells were cultured in three-dimension culture model using collagen gel as frame, and the change of phenotype of ectomesenchymal cells were observed and detected by phase-contrast microscopy and immunohistochemistry after the cells had been treated by 10 ng/ml of bFGF or/and 100 ng/ml IGF-1. Results: 4 days after treatment by bFGF and IGF-1, the cells appeared to be odontoblast-like cells aligned parallelly and polarized with long cytoplasmic processes attached to one end of the cell body.The cells were positive for DSP expression. However, the cells were DSP negative and aligned disorderly in other groups. Conclusion: Ectomesenchymal cells can be induced to differentiate to odontoblast-like cells in three-dimension culture model with the treatment by bFGF and IGF-1.