Preparation and antibacteria activity of crude mutacin from clinical isolate of Mutans streptococci / 实用口腔医学杂志

ABSTRACT

Objective:To obtain crude mutacin produced by clinical isolate of Mutans streptococci (Ms) and to test its antibacteria activity. Methods:The mutacin in supernatant of in vitro cultured clinical isolate of Ms was extracted by chloroform, the antibacteria activity and heat stability of the crude extract were tested by bacteria culture technique. Results:Crude extract of protein was obtained fom clinical isolate of Ms. 10 ml of the liquid extract produced a 19 mm inhibitory zone in cultrue dish, indicating its antibacteria activity. The activity could maintain in 80 ℃ for 120 min, indicating its heat stability. Conclusion: The crude extract can represent the antibacteria activity and heat stability of mutacin.