Mycoplasma in various clones of cells in the production and verification of poliomyelitis vaccine in the year 2002

ABSTRACT

Four line of cells Hep2, Vero, MA104 and JVKT are used for quality control and a line of monkey kidney cells used for production of poliomyelitis vaccine. Contaminated mycoplasma was tested by culture method and immu-mark mycotest. Same results were given 50% of Hep 2 cells and 66.6% of MA104 were contaminated with mycoplasma. There is not any controlled lot of JVKT. Vero and monkey kidney which was contaminated