Lipocalin-type prostaglandin D synthase:preparation of monoclonal antibodies and localization on human sperm / 中国免疫学杂志

ABSTRACT

Objective:To prepare monoclonal antibodies against lipocalin type prostaglandin D synthase and to explore the distribution of lipocalin type prostaglandin D synthase on human sperm.Methods:BALB/c mice were immunized with lipocalin type prostaglandin D synthase,monoclonal antibodies were prepared by hybridoma technique,the biological characteristics of antibodies were analyzed by ELISA and Western blot,the distribution of lipocalin type prostaglandin D synthase on sperm were performed by immunohistochemistry.Results:A strain of hybridoma cell was obtain,and the IgG isotype of the antibody was identified as IgG1,with affinity constat(K) of 2?108 and titer over 103.Western blot demonstrated specific recognition of lipocalin type prostaglandin D synthase by the obtained monoclonal antibody.The result of immunohistochemistry displayed that lipocalin type prostaglandin D synthase was mainly distributed on the surface of human sperm.Conclusion:A hybridoma cell secreting anti-lipocalin type prostaglandin D synthase monoclonal antibodies stably was established.On the surface of human sperm,there is lipocalin type prostaglandin D synthase's distribution. [