The growth-inhibitory effect of endometrial carcinoma cell line HEC-1A induced by somatostatin receptor family / 西安交通大学学报(医学版)

ABSTRACT

Objective To study the expression of somatostatin receptor family in endometrial carcinoma cell line HEC-1A, as well as the growth and apoptosis of HEC-1A cell line when it was treated with somatostatin analogue RC-160. Methods RT-PCR was used to examine the existence of somatostatin receptors on the HEC-1A cells. The anti-proliferative effect of RC-160 on the growth of HEC-1A cells was detected by using BrdU incorporation test. TUNEL staining was used to find out whether apoptosis was involved in the inhibitory process. Results All the five somatostatin receptor subtypes were expressed in HEC-1A cells. RC-160 inhibited the HEC-1A cell growth stimulated by serum in a dose-dependent manner. The effect was maximal at the concentration of 10 -5 mol/L after 48 hours' treatment. No apoptosis was detected. Conclusion Somatostatin analogue RC-160 can inhibit the proliferation of endometrial carcinoma cell line HEC-1A through binding to the somatostatin receptors on the cells. It seems that apoptosis is not the major cause of the inhibition.