The role of transcription factor NF- ?B in vascular endothelial cell impairment induced by high glucose,TNF-? and IL-1? / 中华内分泌代谢杂志

ABSTRACT

Objective To investigate the role of NF-?B in high glucose (HG) and cytokines (TNF-? and IL-1?)-induced impairment of ECV-304 cells (vascular endothelial cell line). Methods Recombinant adenovirus containing NF-?B supper-repressor I?B?M with mutant I?B? was constructed. Western blot, electrophoretic mobility shift assay (EMSA) and thiazolyl blue viability assay were applied in this study. Results TNF-?-induced I?B? degradation and NF-?B activation (P