The effect of low-body weight combined with T~(149)-C and A~(163)-G polymorphism of osteoprotegerin promoter region on osteoporosis / 中华老年医学杂志

ABSTRACT

Objective To investigate the effect of T149-C and A163-G polymorphism of osteoprotegerin promoter region combined with low-body weight on bone mineral density (BMD) of postmenopausal osteoporosis women and postmenopausal healthy women. Methods Seventy-three postmenopausal osteoporosis women and 61 postmenopausal healthy women were enrolled. The shifted patterns were searched from randomly selected 25 samples by SSCP-PCR and their sequences were determined by cycle sequencing. The T149-C and A163-G polymorphisms were determined by PCR-RFLP. BMD of lumbar spines and femoral neck, Ward and trochanteric areas were measured by dual energy X-ray absorptiometry. Results T149-C and A163-G polymorphisms in the postmenopausal osteoporosis women and the postmenopausal healthy women were through Hardy Weinberg equilibrium. Both single and combined genotype frequencies of the T149-C and A163-G polymorphism did not show any difference between postmenopausal osteoporosis women and postmenopausal healthy women. The BMD levels of the postmenopausal osteoporosis women were significantly lower than those of the postmenopausal healthy women in lumbar spines and femoral neck, and BMI levels of the postmenopausal osteoporosis women was significantly lower than those of the postmenopausal healthy women. Conclusions The T149-C and A163-G polymorphism has no synergistic effect on bone mass in both the postmenopausal osteoporosis women and the postmenopausal healthy women. The single and combined genotypes of the T149-C and A163-G polymorphism may not be used as genetic markers in predicting their risk of developing osteoporosis in Chinese women of the Han nationality, but may be susceptible gene of osteoporosis.