Detection of p53 Gene Mutation in Endemic Arsenism Patients by PCR-SSCP / 环境与健康杂志
Journal of Environment and Health
; (12)1989.
Article
em Zh
| WPRIM
| ID: wpr-544173
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study whether arsenic can induce p53 gene mutation in the lymphocytes in the peripheral blood and the skin cancer tissue of the patients with endemic arsenism in Xinjiang. Methods PCR-SSCP silver staining technique was employed to detect the mutation of exon E5-9 of p53 gene. Results Gel electrophoresis imaging demonstrated Exon E6, 7 of p53 gene appeared abnormal strips, strip absence and strip shift. Conclusion Arsenic can induce p53 gene mutation, the mutation sites located on exon E6,7.
Texto completo:
1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Journal of Environment and Health
Ano de publicação:
1989
Tipo de documento:
Article