Effects of atrial natriuretic peptide preconditioning on hepatic ischemia-reperfusion injury in rats / 中国现代普通外科进展

ABSTRACT

Objective:To investigate the protective effects of matrine(MT) preconditioning against hepatic ischemia-reperfusion injury in rats. Methods:Fifty male SD rats were randomly allocated into 5 groupssham operation(SO) group,ischemia-reperfusion (NS) group and MT preconditioning groups (of 3.75,7.5,15 mg/kg). Rats of NS and MT groups were injected normal saline or MT,respectively; and 15 min later,the pedicales of the left and median liver lobes were clampped for 45 minutes,and reperfused for 40 min. The portal trial were only separated but not obstructed in SO rats. After reperfusion,the serum activity of ALT,AST and LDH was determined,the activity of SOD and content of MDA in liver tissue were examined,and histopathological changes of liver were observed. Results:Compared with those of NS group,the serum levels of ALT,AST,LDH,and the content of MDA in liver tissue of MT groups were lower,and the activity of SOD was higher(P