SYNTHESIS AND SECRETION OF HUMAN IFN-?B IN SACCHAROMYCE CEREVISIAE / 第二军医大学学报

ABSTRACT

Modified HindⅢ fragment of IFN-?B gene was treated by nuclease S1 inserted into the HindⅢ site of plasmid YFD18 and fused with leader sequence of ?-factor gene,Resultant plasmid YFD33 was transformed into ?-type yeast strain Y33.The transforments could synthesize and secrete IFN-?B.More than 1.4?107 units/liter of interferon antiviral activity were in the medium and about 1.1?107 units/liter were in the cell.