Regulation on function and maturation of plasmacytoid dendritic cells by Astragalus polysaccharide / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2000.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-548876
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To explore the mechanism for immunomodulatory activity of Astragalus polysaccharide (APS) with peripheral blood mononuclear cells(PBMC)-derived plasmacytoid dendritic cells (pDCs) from healthy volunteers.Methods:
Healthy volunteers-derived pDCs were sorted by flow cytometry,then incubated with APS (0m50,100,200 mg/L ).After 24 hours,the concentration of IFN-?,TNF-?,IL-6 was detected using ELISA.After 5 days,the cultured cells were collected and analyzed by flow cytometry,light microscope and electron microscope scanning.Results:
APS-treated pDCs secreted higher level of IFN-?,TNF-?,IL-6.APS could promote differentiation of pDCs to dendritic cells (DCs) which displayed more matured morphology and immunophenotypes compared to the untreated-pDCs.Conclusion:
APS could increase the immune function of pDCs,promote differentiation and maturation of pDCs.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2000
Tipo de documento:
Artigo