The Basic Characteristics of Low-affinity Glucocorticoid Binding Sites / 第二军医大学学报
Article
em Zh
| WPRIM
| ID: wpr-550514
Biblioteca responsável:
WPRO
ABSTRACT
Scatchard, pseudoscatchard and competitive analyses showed that there were two glucocorticoid (GC) binding sites in rat hepatic cytosol: high-affinity glucocorticoid binding sites (HAGS) and low-affinity glucocorticoid binding sites (LAGS). Pseudoscatchard analysis showed the dissociation constant (Kd) of LAGS was 4.1+1.4umol/L (n=4). The inactivation of LAGS in vitro was slower" than that of HAGS. LAGS and HAGS could not be separated by DEAE-cellulose chfomatography. HAGS were saturable and had steroid specificity for glucocorticoid just as the classic glucocorticoid receptor. Triamcinolone acetonide, RU486 and RU26988 competed equally well to LAGS while aldosterone, progesterone, estradiol and testosterone did not compete with LAGS. These results suggest that LAGS have steroid specificity for glucocorticoids. The biological actions of LAGS await fur ther study.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
1981
Tipo de documento:
Article