Highly effective transformation of Cryptococcus neoformans / 第二军医大学学报
Article
em Zh
| WPRIM
| ID: wpr-553307
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To establish a highly effective transformation method of Cryptococcus neoformans. Methods: Special reagents was used to make C. neoformans take in external DNA under given condition. This chemical transformation result was compared with that of electrotransformation. The feasibility of this chemical transformation was tested by plasmid stability test. Results: The efficiency of this chemical transformation was more than 103 transformants/?g plasmid DNA, far more than that of the traditional electrotransformation. Conclusion: An appropriate transformation method is established for C. neoformans transformation, which has high transformation efficiency.
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2000
Tipo de documento:
Article